Product Introduction
Formalin Fixed Paraffin Embedded (FFPE) treatment can preserve the original pathophysiological state of tissue cells for a relatively long period. Due to its relatively simple and mature production process, low cost, and simple storage conditions (room temperature is sufficient), it has been widely used in biological research and clinical pathology testing for a long time and is currently the gold standard for long-term preservation of pathological samples. The current archive of FFPE samples is enormous, containing a wealth of valuable pathophysiological information, which urgently needs further exploration.
Formaldehyde fixation and paraffin embedding lead to extensive molecular cross-linking of biomolecules such as proteins and nucleic acids in tissue cells, significantly reducing the efficiency of protein extraction, protease digestion, and the utilization rate of fragmented particle spectroscopy (identification efficiency) in FFPE samples. Our method combines highly efficient FFPE protein extraction technology with the powerful Astral Zoom mass spectrometry platform, which can greatly increase the depth of sample identification, effectively promoting the utilization of FFPE samples and research on related diseases, and fully exploring the basic research and clinical value of FFPE samples.
Technical Process
Case Studies
Results presentation of the FFPE proteomics project
Top left diagram: Protein count between groups; Top right diagram: Intergroup expression of proteins; Bottom diagram: Molecular pathway enrichment analysis
Sample Submission Requirements
- Sample
- 3 slices/sample, ~1cm^2/slice
- Quantity
- Repeated ≥15 times
| Sample | Quantity |
|---|---|
| 3 slices/sample, ~1cm^2/slice | Repeated ≥15 times |
References
Pu J et al Highly Accurate and Robust Absolute Quantification of Target Proteins in Formalin-Fixed Paraffin-Embedded (FFPE) Tissues by LC-MS Anal Chem, 2023 Jan 17;95(2):924-934.
Soni RK rotocol for deep proteomic profiling of formalin-fixed paraffin-embedded specimens using a spectral library-free approach STAR Protoc, 2023 Sep 15;4(3):102381.
Mitsa G et al A Non-Hazardous Deparaffinization Protocol Enables Quantitative Proteomics of Core Needle Biopsy-Sized Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens Int J Mol Sci, 2022 Apr 18;23(8):4443.
Shenzhen Wininnovate Bio Co., Ltd.
Innovative mass spectrometry and AI technologies provide protein and metabolite mass spectrometry multi-omics solutions for life science research, empowering the growth of the biotechnology, pharmaceutical, and healthcare industries.
