Product Overview
Characterization of active constituents in traditional Chinese medicine (TCM) is a core aspect of modern TCM research. The chemical composition of TCM is extremely complex; therefore, the rapid and accurate identification of its active components is crucial for elucidating the pharmacodynamic material basis, controlling TCM quality, and revealing its mechanisms of action.
Wininnovate Bio, leveraging its advanced ultra-high performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) platform and powerful bioinformatics tools, has launched a one-stop characterization service for active constituents in TCM. This service is specifically designed for the analysis of raw TCM materials and compound preparations, aiming to systematically analyze the chemical composition of TCM and provide a solid data foundation for TCM quality control, serum pharmacology, and network pharmacology research.
Technological Advantages
1. Exclusive Self-Built Database for More Precise Identification: Possessing a self-built Wininnovate-TCM1.0 Traditional Chinese Medicine (TCM) database, covering over 500 commonly used TCMs in clinical practice and including more than 13,000 compounds. The database's comprehensive information greatly enhances the breadth and accuracy of component identification in complex TCM systems.
2. High-Resolution Mass Spectrometry Platform for More Comprehensive Coverage: Utilizing high-resolution mass spectrometers such as Orbitrap, combined with longer gradient elution times and data-dependent acquisition modes, ensures effective capture and separation of low-abundance and trace active ingredients.
3. Professional End-to-End Services for Deeper Analysis: From sample pretreatment and data acquisition to compound identification, professional manual verification is provided. Seamless integration with network pharmacology analysis is also available, offering in-depth data mining services such as target prediction, pathway enrichment, and construction of "component-target-pathway" network diagrams.
Technical Process
Key Processing Nodes
1. Sample Pretreatment: Differentiated extraction schemes are employed based on sample dosage forms (e.g., herbal powders, compound decoctions, etc.) to ensure efficient extraction of target components.
2. Data Acquisition: The UPLC-HRMS platform is used with DDA acquisition mode, and samples are injected separately in positive and negative ion modes to maximize coverage of various chemical components in traditional Chinese medicine.
3. Metabolite Identification: Based on the precise mass number, isotope distribution, and secondary fragment spectra of compounds, accurate matching is performed with the self-built Wininnovate-TCM1.0 database, supplemented by manual verification to ensure reliable qualitative results.
4. Bioinformatics Analysis: Principal Component Analysis (PCA), differential component screening, cluster heatmaps, and other analyses are provided, and personalized network pharmacology analysis (target prediction, GO/KEGG enrichment, molecular docking, etc.) is supported.
Case Studies
Gao, Y., Liang, X., Zhang, N., Zhang, X., Li, X., & Gao, W. (2024). Untargeted Profiling of Shenfu Qiangxin Pills Based on High‐Resolution Mass Spectrometry and Absolute Quantitation of Multiple Components Using Parallel Reaction Monitoring. Journal of Separation Science, 47(23), e70025
Sample Submission Requirements
- Sample Type
- Chinese medicinal herbs / processed slices
- Minimum Sample Quantity
- ≥5 g
- Storage & Transportation Conditions
- Store at room temperature in a dry place, away from light; transport by express courier.
- Precautions
- It is recommended to pulverize the material and send it for testing. Please provide information on the identification of the medicinal material.
- Sample Type
- Chinese medicine powder/granules/capsules
- Minimum Sample Quantity
- ≥5 g
- Storage & Transportation Conditions
- Store at room temperature in a dry place; transport by express delivery.
- Precautions
- Simply send the contents powder for testing.
- Sample Type
- Compound decoction/oral liquid
- Minimum Sample Quantity
- ≥20 mL
- Storage & Transportation Conditions
- Store frozen at -20℃; transport with dry ice.
- Precautions
- It is recommended to repackage the product, with each tube not exceeding 2/3 of its volume, to avoid freezing and cracking.
| Sample Type | Minimum Sample Quantity | Storage & Transportation Conditions | Precautions |
|---|---|---|---|
| Chinese medicinal herbs / processed slices | ≥5 g | Store at room temperature in a dry place, away from light; transport by express courier. | It is recommended to pulverize the material and send it for testing. Please provide information on the identification of the medicinal material. |
| Chinese medicine powder/granules/capsules | ≥5 g | Store at room temperature in a dry place; transport by express delivery. | Simply send the contents powder for testing. |
| Compound decoction/oral liquid | ≥20 mL | Store frozen at -20℃; transport with dry ice. | It is recommended to repackage the product, with each tube not exceeding 2/3 of its volume, to avoid freezing and cracking. |
General Requirements: 1. Sample Identification: Please clearly label the sample number using a waterproof label and include a sample information sheet. 2. Transportation: Please use sufficient dry ice (5-10 kg/day) to ensure the samples arrive still encased in dry ice.
References
Gao, Y., Liang, X., Zhang, N., Zhang, X., Li, X., & Gao, W Untargeted Profiling of Shenfu Qiangxin Pills Based on High‐Resolution Mass Spectrometry and Absolute Quantitation of Multiple Components Using Parallel Reaction Monitoring Journal of Separation Science, 2024;47(23), e70025.
Shenzhen Wininnovate Bio Co., Ltd.
Innovative mass spectrometry and AI technologies provide protein and metabolite mass spectrometry multi-omics solutions for life science research, empowering the growth of the biotechnology, pharmaceutical, and healthcare industries.
